BioBoard/Documentation/Optical loss

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(Created page with '=Introduction to absorbance= =Building an NIR probe= ==What you need== ==How to build it== ==Things to keep in mind== =Interfacing and measuring= =Calibrating= =Making it …')
 
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=Introduction to absorbance=
 
=Introduction to absorbance=
 +
 +
Measuring optic loss - different techniques
 +
Optic loss as a proxy for biomass
  
 
=Building an NIR probe=
 
=Building an NIR probe=
 +
 +
Light source and light sensor pairing
 +
Discussion of pros/cons of different source/sensor pairs
  
 
==What you need==
 
==What you need==
 +
 +
IR LED
 +
Phototransistor
 +
2 5kΩ resistors
 +
Wire
 +
Soldering iron + solder
 +
¾" acrylic tube
 +
¾" acrylic discs
 +
Acrylic cement (thick)
 +
1" PVC pipe
 +
Aquarium glue/hot glue
 +
Optional: cell-phone motor (BubbleShaker Technology)
  
 
==How to build it==
 
==How to build it==
 +
 +
Cutting acrylic and PVC
 +
Drilling holes
 +
Soldering wires
 +
Fixing diodes
 +
Glueing discs on tubes
 +
Plugging tubes
 +
Covering it up
  
 
==Things to keep in mind==
 
==Things to keep in mind==
 +
 +
Aquarium glue vs hot glue
 +
Biologically inert materials
 +
Food safety
  
 
=Interfacing and measuring=
 
=Interfacing and measuring=
 +
 +
Arduino sketch should go here...
  
 
=Calibrating=
 
=Calibrating=
 +
 +
How to find out whether your measurements are accurate
 +
How to adjust (distance, resistance)
  
 
=Making it cooler=
 
=Making it cooler=
 +
 +
Multi-channel measurements
  
 
=Geeking out=
 
=Geeking out=
 +
 +
Reduction of light passing through a mass
 +
Absorbance vs. scattering
  
 
=Links=
 
=Links=
 +
 +
Optek

Revision as of 22:20, 26 April 2011

Contents

Introduction to absorbance

Measuring optic loss - different techniques Optic loss as a proxy for biomass

Building an NIR probe

Light source and light sensor pairing Discussion of pros/cons of different source/sensor pairs

What you need

IR LED Phototransistor 2 5kΩ resistors Wire Soldering iron + solder ¾" acrylic tube ¾" acrylic discs Acrylic cement (thick) 1" PVC pipe Aquarium glue/hot glue Optional: cell-phone motor (BubbleShaker Technology)

How to build it

Cutting acrylic and PVC Drilling holes Soldering wires Fixing diodes Glueing discs on tubes Plugging tubes Covering it up

Things to keep in mind

Aquarium glue vs hot glue Biologically inert materials Food safety

Interfacing and measuring

Arduino sketch should go here...

Calibrating

How to find out whether your measurements are accurate How to adjust (distance, resistance)

Making it cooler

Multi-channel measurements

Geeking out

Reduction of light passing through a mass Absorbance vs. scattering

Links

Optek

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