BioBoard/Documentation/Optical loss

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Contents

Introduction to absorbance

Measuring optic loss - different techniques

Optic loss as a proxy for biomass

Building an NIR probe

Light source and light sensor pairing

Discussion of pros/cons of different source/sensor pairs

What you need

  • IR LED
  • Phototransistor
  • 2 5kΩ resistors
  • Wire
  • Soldering iron + solder
  • ¾" acrylic tube
  • ¾" acrylic discs
  • Acrylic cement (thick)
  • 1" PVC pipe
  • Aquarium glue/hot glue

Optional: cell-phone motor (BubbleShaker Technology)

How to build it

Cutting acrylic and PVC

Drilling holes

Soldering wires

Fixing diodes

Glueing discs on tubes

Plugging tubes

Covering it up

Things to keep in mind

Aquarium glue vs hot glue

Biologically inert materials

Food safety

Interfacing and measuring

Arduino sketch should go here...

Calibrating

How to find out whether your measurements are accurate

How to adjust (distance, resistance)

Making it cooler

Multi-channel measurements

Geeking out

Reduction of light passing through a mass

Absorbance vs. scattering

Links

Optek

Personal tools